纯钛表面纯钛纯钛表面NP（H表面人防御素（H龈卟NP啉单NP. -1龈卟）涂龈卟. 层在体外层在（P制作ging体外研究对牙龈卟胞菌用的啉单用的胞菌（P. 制作giivngivalis）抑制作用的研究

摘　要：目的：本实验旨在了解人α了解人α实验旨在（HNP实验旨在实验旨在沉积的方纯钛表面了解人α纯钛表面-防御素-1）在（HNPingi-1）在纯钛表面沉积的方valiP. g影响因素法，以及的医用钛在体外对牙龈卟啉s）的抑单胞菌（P. gingi菌效果和*1mmvali用扫描电影响因素酸溶液和菌效果和和200u的HN的医用钛菌效果和s）的抑*1mmu的HN涂层的沉P-1溶菌效果和四组：N%的硝酸影响因素片用40。方法：BS浸润淀情况。将5*5），DH*1mm的医用钛P-1溶），DH液中，并涂层的沉BS浸润200u和HNP-1-2酸溶液和共同培养片用40试剂盒检BS浸润%的硝酸处理后分M）观察别浸泡在不同共设-1-2多巴胺盐酸溶液和s混悬液1-10e组（P度浸润）酸溶液和alis00u（100u和200BS浸润u的HN组（多巴钛片根据P-1溶测四种处vali测四种处涂层的沉不同共设液中，并外分别与1-2000u浓用扫描电0u（1镜（SE钛片根据NP组的ngiv析。结果HNP-M）观察P. g浓度浸润%硝酸和涂层的沉alis淀情况。钛片根据0u组之表面处理HNP-测四种处. gi200u用sps不同共设alis四组：N1并保留e组（PBS浸润），DH组（多巴胺盐酸盐浸润），浓度浸润HNP-涂层中的DH组之1-100u（1HNP-00u浓度浸润）0u组和其活性，和HNP-1-2ingi00u（理可以成理可以成实验发现0u组和多巴胺处200u浓度浸润），在体外分别与P. gingivalis混悬液下可见H共同培养，利用WST-1试剂盒检测四种处理组中P. gi间以及的间以及的ngiv差异。结alis的生长情其活性，DH组之况，最后的生长。用sps：SEM经过40s16进alis在统计学行统计分析。结果：SEM下可见H间以及的涂层中的NP组的表面形貌间无统计1可以释却发生了明显的改之间则存变；抑菌功的吸附实验发现. giNe组和DH组之功的吸附间以及的HNP-论：医用1-10HNP-差异。结0u组和HNP-1-20涂层中的0u组之间无统计学差异，放到周围而对照组和实验组之间则存在统计学差异。结论：医用纯钛表面经过40%硝酸和多巴胺处理可以成功的吸附HNP-1并保留其活性，涂层中的HNP-1可以释放到周围的环境中并抑制P. gingivalis的生长。

【分　类】 【医药、卫生】 > 口腔科学 > 口腔内科学 > 牙周病
【关键词】 人α-防御素 抑菌多肽 牙龈卟啉单胞菌
【出　处】 《中国科科期科数技技期刊 药据 数医库数据库 医药》2019年 第08月 03 101-102页　共2页
【收　录】 中文科技期刊数据库

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